DIABETES, VOLUME 45, SUPPLEMENT 1, JANUARY 1996, PAGES S24-S28
Immunolocalization of Bradykinin B2 Receptors on
Skeletal Muscle Cells
Carlos D. Figueroa, Gunther Dietze, and Werner Muller-Esterl
The kallikrein-kinin system has been implicated in the inflammatory process, blood pressure
regulation, renal homeostasis, and glucose utilization. The effects of kallikrein and kinin on
glucose uptake by the skeletal muscle are well established; however, the occurrence and the
cellular distribution of the kinin receptor(s) mediating these effects in the striated muscle
are unknown. Using anti-peptide antibodies raised against the predicted intra- and
extracellular domains of the B2 receptor and the peroxidase/antiperoxidase system, we have
been able to detect tbe B2 receptor on the plasma membrane of striated skeletal muscle cells
of the rat hindlimb. A strong immunostaining appeared as a rim of immunoreactive material
located on the periphery of striated muscle cells. Cross-sectioned and longitudinally
sectioned cells revealed a similar staining pattern. Alternatively, the immunostaining with
specific antibodies to tissue kallikrein and to T-kininogen did not yield a significant staining
of the striated muscle cells. Localization of the B2 receptor on the surface of striated muscle
cells provides a structural basis for the hypothesized physiological functions of the kinin
system in the skeletal muscle. Diabetes 45 (Suppl. 1):S24-S28, 1996
Copyright © 1996 American Diabetes Association
Last updated: 5/30/96
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