DIABETES, VOLUME 45, SUPPLEMENT 1, JANUARY 1996, PAGES S88-S92

Control of Energy Metabolism During Muscle Contraction

George K. Radda

Skeletal muscle energetics can be studied noninvasively at rest, during exercise, and in recovery using phosphorus nuclear magnetic resonance (³¹P-NMR). In resting musle, inorganic phosphate (P_i) and total cellular phosphate concentration are regulated by Na⁺-dependent P_i transport. Insulin was shown to stimulate P_i uptake in G-8 muscle cells, in isolated rat soleus muscle, and in human muscle in vivo under conditions of hyperinsulinemic-euglycemic clamp. The relationship between plasma P_i and intracellular muscle P_i was examined in a group of patients with elevated plasma P_i resulting from renal failure. The total creatine content of muscle cells is controlled by an active creatine uptake in which B₂-receptor stimulation and the activity of the Na⁺-K⁺TPase play a significant role. Recovery after exercise is entirely oxidative; the rate of ATP synthesis is largely controlled by ADP, the concentration of which is deternined by the creatine kinase equilibrium that includes he concentration of H⁺. At the onset of aerobic dynamic exercise, ATP is maintained largely by glycolysis, producing lactic acid, and by phosphocreatine breakdown. After vasodilation, ATP synthesis becomes predominantly oxidative. The above processes can be quantitatively evaluated by 31P-NMR. Diabetes 45 (Suppl. l):S88-S92, 1996

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