DIABETES, VOLUME 45, SUPPLEMENT 1, JANUARY 1996, PAGES S93-S98

Nuclear Magnetic Resonance Studies of Muscle and Applications to Exercise and Diabetes

R. G. Shulman, D. L. Rothman, and T. B. Price

Natural-abundance ¹³C nuclear magnetic resonance (NMR) spectroscopy is a noninvasive technique that en-ables in vivo assessments of muscle and/or liver glycogen concentrations. When directly compared with the tradi-tional needle biopsy technique, NMR was found to be more precise. Over the last several years, we have devel-oped and used ¹³C-NMR to obtain information about human glycogen metabolism both under conditions of altered blood glucose and/or insulin and with exercise. Because NMR is noninvasive, we have been able to obtain more data points over a specified time course, thereby dramatically improving the time resolution. This im-proved time resolution has enabled us to document sub-tleties of the resynthesis of muscle glycogen after severe exercise that have not been observed previously. An added advantage of NMR is that we are able to obtain information simultaneously about other nuclei, such as ³¹p. With interleaved ¹³C- and ³¹P-NMR techniques, we have been able to follow simultaneous changes in muscle glucose-6-phosphate and muscle glycogen. In this article, we review some of the work that has been reported by our laboratory and discuss the relevance of our findings for the management of diabetes. Diabetes 45 (Suppl. 1):S93--S98, 1996

Copyright © 1996 American Diabetes Association

Last updated: 5/30/96
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